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Anti-Escherichia coli ptsH/hpr Recombinant Antibody (Jel42) (JN867013)

Anti-Escherichia coli ptsH/hpr Recombinant Antibody (Jel42)
Anti-Escherichia coli ptsH/hpr Recombinant Antibody (Jel42)
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Overview
Catalog No.JN867013
Species reactivityEscherichia coli (strain K12)
ApplicationsELISA
Host speciesHuman
IsotypeIgG1, kappa
Clone IDJel42
Clonality Monoclonal
Target ptsH, hpr, Phosphocarrier protein HPr, Histidine-containing protein
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P0AA04
RRID Anti-Escherichia coli ptsH/hpr Antibody (Jel42) (abinScience Cat# JN867013, RRID:AB_3727033)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Phosphocarrier protein HPr (ptsH) is a ~9 kDa protein. General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA domain.

Note For research use only.
Images
  • Anti-Escherichia coli ptsH/hpr Recombinant Antibody (Jel42)

    SDS-PAGE

    SDS-PAGE for Anti-Escherichia coli ptsH/hpr Antibody (Jel42)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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