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Anti-Etanercept Polyclonal Antibody (AF879044)

Anti-Etanercept Polyclonal Antibody
Anti-Etanercept Polyclonal Antibody
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  • 50ug
  • 100ug
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Overview
Catalog No.AF879044
Description
Anti-Etanercept Polyclonal Antibody (AF879044) is a rabbit polyclonal antibody detecting Etanercept in ELISA.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
ApplicationsELISA
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen Etanercept
Target Rabbit polyclonal to Enbrel.
Specificity The product is specific for Etanercept. This antibody serves as an excellent positive control for Etanercept immunogenicity (ADA) assays.
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession CAS: 185243-69-0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage The product can be stored for 2 - 3 weeks at 2 to 8°C or for up to 12 months at -20°C. Avoid repeated freezing and thawing cycles.
BackgroundEtanercept with trade name Enbrel, is an FDA-approved drug to treat rheumatoid arthritis, juvenile idiopathic arthritis and psoriatic arthritis, plaque psoriasis and ankylosing spondylitis. Etanercept is a fusion protein made from the combination of two naturally occurring soluble human 75-kilodalton TNF receptors linked to an Fc portion of an IgG1. Etanercept treats autoimmune diseases by interfering with tumor necrosis factor-alpha (TNFα, a soluble inflammatory cytokine) by acting as a TNFα inhibitor.
Note For research use only.
Images
  • Anti-Etanercept Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Etanercept polyclonal antibody at 1ug/mL on recombinant Etanercept (Catalog No. HF879296).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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