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Anti-ETFA Polyclonal Antibody (HB633014)

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Overview
Catalog No.HB633014
Description
Anti-ETFA Polyclonal Antibody (HB633014) is a rabbit polyclonal antibody detecting ETFA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ETFA (Ser52-Thr295).
Target Alpha-ETF, ETFA, Electron transfer flavoprotein subunit alpha, mitochondrial
Purification Purified by antigen affinity column.
Accession P13804
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Electron transfer flavoprotein subunit alpha, mitochondrial (ETFA) is a ~35 kDa protein. Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.

1. Bross, P. et al. (1999) Molecular genetics and metabolism 67, 138-47. PMID: 10356313
2. Toogood, HS. et al. (2004) The Journal of biological chemistry 279, 32904-12. PMID: 15159392
3. Toogood, HS. et al. (2005) The Journal of biological chemistry 280, 30361-6. PMID: 15975918
4. Floyd, BJ. et al. (2016) Molecular cell 63, 621-632. PMID: 27499296
5. Salazar, D. et al. (1997) The Journal of biological chemistry 272, 26425-33. PMID: 9334218
6. Olsen, RK. et al. (2003) Human mutation 22, 12-23. PMID: 12815589
7. Freneaux, E. et al. (1992) The Journal of clinical investigation 90, 1679-86. PMID: 1430199
8. Indo, Y. et al. (1991) American journal of human genetics 49, 575-80. PMID: 1882842
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ETFA Polyclonal Antibody

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