Anti-EYA2 Polyclonal Antibody (HC008014)

Name: Anti-EYA2 Polyclonal Antibody
Brand: abinScience
SKU: HC008014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC008014

Description

Anti-EYA2 Polyclonal Antibody (HC008014) is a rabbit polyclonal antibody in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human EYA2 (Glu266-Leu538).

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O00167, Q58DB6, O08575, Q9YHA0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein phosphatase EYA2 is a ~59 kDa protein. Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1.

1. Fougerousse, F. et al. (2002) Journal of muscle research and cell motility 23, 255-64. PMID: 12500905

2. Patrick, AN. et al. (2013) Nature structural & molecular biology 20, 447-53. PMID: 23435380

3. Krishnan, N. et al. (2009) The Journal of biological chemistry 284, 16066-16070. PMID: 19351884

Note

For research use only

Images

References

Datasheet

Anti-EYA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EYA2 Polyclonal Antibody (HC008014)

Overview

Images

References

Datasheet

SDS