Anti-FAHD1 Polyclonal Antibody (HB329014)

Name: Anti-FAHD1 Polyclonal Antibody
Brand: abinScience
SKU: HB329014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB329014

Description

Anti-FAHD1 Polyclonal Antibody (HB329014) is a rabbit polyclonal antibody detecting mitochondrial; OAA decarboxylase; ODx; 4.1.1.112; Acylpyruvase FAHD1; 3.7.1.5; Fumarylacetoacetate hydrolase domain-containing protein 1; FAH domain-containing protein 1; YisK-like protein; FAHD1; C16orf36; YISKL in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human FAHD1 (Ala25-Tyr221).

Target

Oxaloacetate decarboxylase, mitochondrial; OAA decarboxylase; ODx; 4.1.1.112; Acylpyruvase FAHD1; 3.7.1.5; Fumarylacetoacetate hydrolase domain-containing protein 1; FAH domain-containing protein 1; YisK-like protein; FAHD1; C16orf36; YISKL

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q6P587, Q2HJ98, Q6AYQ8, Q8R0F8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Oxaloacetate tautomerase FAHD1, mitochondrial is a ~24 kDa protein. Tautomerase that converts enol-oxaloacetate, a strong inhibitor of succinate dehydrogenase, to the physiological keto form of oxaloacetate. It is thereby required to maximize aerobic respiration efficiency by preventing succinate dehydrogenase inhibition. Also acts as a weak oxaloacetate decarboxylase (ODx), catalyzing the decarboxylation of oxaloacetate (OAA) to pyruvate and CO(2), and as such is likely a regulatory enzyme in the TCA cycle. Also displays acylpyruvase activity, being able to hydrolyze acetylpyruvate and fumarylpyruvate in vitro. Exhibits only a weak hydrolase activity on methylacetopyruvate and acetylacetone, and no activity toward acetoacetyl-CoA.

1. Zmuda, AJ. et al. (2024) Nature communications 15, 846. PMID: 38287013

2. Pircher, H. et al. (2015) The Journal of biological chemistry 290, 6755-62. PMID: 25575590

3. Weiss, AKH. et al. (2018) The Biochemical journal 475, 3561-3576. PMID: 30348641

4. Pircher, H. et al. (2011) The Journal of biological chemistry 286, 36500-8. PMID: 21878618

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-FAHD1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Mouse kidney lysate
Lane 4: Rat kidney lysate
Lane 5: Mouse liver lysate
Lane 6: Rat liver lysate

References

Datasheet

Anti-FAHD1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-FAHD1 Polyclonal Antibody (HB329014)

Overview

Images

References

Datasheet

SDS