Anti-FCN1 Polyclonal Antibody (HT184014)

Name: Anti-FCN1 Polyclonal Antibody
Brand: abinScience
SKU: HT184014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT184014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human FCN1 (Gly130-Ala326).

Target

Ficolin-alpha, FCNM, Ficolin-A, FCN1, Ficolin-1, Collagen/fibrinogen domain-containing protein 1, M-ficolin

Purification

Purified by antigen affinity column.

Accession

O00602

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ficolin-1 (FCN1) is a ~35 kDa protein. Extracellular lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Specifically recognizes and binds carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8.

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1. Liu, Y. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 3150-6. PMID: 16116205

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2. Garlatti, V. et al. (2007) The Journal of biological chemistry 282, 35814-20. PMID: 17897951

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3. Gout, E. et al. (2010) The Journal of biological chemistry 285, 6612-22. PMID: 20032467

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4. Honoré, C. et al. (2010) Journal of leukocyte biology 88, 145-58. PMID: 20400674

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5. Zhang, J. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 6899-910. PMID: 21037097

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Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-FCN1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse liver lysate
Lane 2: Rat liver lysate