Anti-FCoV NP/Nucleoprotein Polyclonal Antibody (VK405014)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK405014

Description

Anti-FCoV NP/Nucleoprotein Polyclonal Antibody (VK405014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Feline coronavirus (strain FIPV WSU-79/1146) (FCoV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant FCoV NP/Nucleoprotein (Met1-Asn377).

Target

Nucleoprotein, Nucleocapsid protein, NC, Protein N, N, 6

Purification

Purified by antigen affinity column.

Accession

P25909

RRID

Anti-FCoV NP/Nucleoprotein Polyclonal Antibody (abinScience Cat# VK405014, RRID:AB_3724979)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoprotein (NP) is a ~42 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-FeCoV-N polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant FCoV NP/Nucleoprotein (Catalog No: VK405012)

References

Datasheet

Anti-FCoV NP/Nucleoprotein Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-FCoV NP/Nucleoprotein Polyclonal Antibody (VK405014)

Overview

Images

References

Datasheet

Quick access

Contact Us