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Anti-FGF8 Polyclonal Antibody (HX070014)

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Overview
Catalog No.HX070014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human FGF8 (Gln23-Arg233).
Target FGF-8, HBGF-8, Heparin-binding growth factor 8, Fibroblast growth factor 8, AIGF, Androgen-induced growth factor, FGF8
Purification Purified by antigen affinity column.
Accession P55075
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Fibroblast growth factor 8 (FGF8) is a ~26 kDa protein. Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Plays a role in neurite outgrowth in hippocampal cells.

1. Olsen, SK. et al. (2006) Genes & development 20, 185-98. PMID: 16384934
2. Zhang, X. et al. (2006) The Journal of biological chemistry 281, 15694-700. PMID: 16597617
3. Ornitz, DM. et al. (1996) The Journal of biological chemistry 271, 15292-7. PMID: 8663044
4. Vantaggiato, C. et al. (2011) Brain : a journal of neurology 134, 1808-28. PMID: 21576111
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-FGF8 Polyclonal Antibody

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