Anti-FHIT Polyclonal Antibody (HB415014)

Name: Anti-FHIT Polyclonal Antibody
Brand: abinScience
SKU: HB415014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB415014

Description

Anti-FHIT Polyclonal Antibody (HB415014) is a rabbit polyclonal antibody detecting P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human FHIT (Met1-Gln147).

Target

Bis(5'-adenosyl)-triphosphatase; 3.6.1.29; AP3A hydrolase; AP3Aase; Adenosine 5'-monophosphoramidase FHIT; 3.9.1.-; Adenylylsulfatase; 3.6.2.1; Adenylylsulfate-ammonia adenylyltransferase; 2.7.7.51; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P49789, O89106, Q1KZG4, Q9JIX3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Bis(5'-adenosyl)-triphosphatase (FHIT) is a ~16 kDa protein. Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2.

1. Trapasso, F. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1592-7. PMID: 12574506

2. Huang, K. et al. (2004) Biochemistry 43, 7637-42. PMID: 15182206

3. Barnes, LD. et al. (1996) Biochemistry 35, 11529-35. PMID: 8794732

4. Lima, CD. et al. (1997) Science (New York, N.Y.) 278, 286-90. PMID: 9323207

5. Brenner, C. et al. (1997) Protein engineering 10, 1461-3. PMID: 9543008

6. Pace, HC. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 5484-9. PMID: 9576908

9. Weiske, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20344-9. PMID: 18077326

Note

For research use only

Images

References

Datasheet

Anti-FHIT Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-FHIT Polyclonal Antibody (HB415014)

Overview

Images

References

Datasheet

SDS