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Anti-FHV-1 glycoprotein B/gB Polyclonal Antibody (VK028014)

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Overview
Catalog No.VK028014
Description
Anti-FHV-1 glycoprotein B/gB Polyclonal Antibody (VK028014) is a rabbit polyclonal antibody detecting herpes simplex virus glycoprotein B (gB) homolog in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityFeline herpesvirus 1 (FeHV-1) (Feline viral rhinotracheitis virus)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant FHV-1 glycoprotein B/gB (Asn165-Thr377).
Target herpes simplex virus glycoprotein B (gB) homolog, glycoprotein B homolog
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession AAB24381.2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
WB 1:500-1:2000
IHC 1:50-1:500
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Envelope glycoprotein B (gB) is a ~102 kDa protein. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. May be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Interacts with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-FHV-1 glycoprotein B/gB Polyclonal Antibody

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