Anti-FIS1 Polyclonal Antibody (HV072014)

Name: Anti-FIS1 Polyclonal Antibody
Brand: abinScience
SKU: HV072014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HV072014

Description

Anti-FIS1 Polyclonal Antibody (HV072014) is a rabbit polyclonal antibody detecting FIS1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Human, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human FIS1 (Met1-Ala117).

Target

Mitochondrial fission 1 protein, FIS1 homolog, Tetratricopeptide repeat protein 11, FIS1, TTC11, hFis1, TPR repeat protein 11

Purification

Purified by antigen affinity column.

Accession

Q9Y3D6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitochondrial fission 1 protein (FIS1) is a ~16 kDa protein. Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event. Also mediates peroxisomal fission. May act when the products of fission are directed toward mitochondrial homeostasis, mitophagy, or apoptosis.

1. James, DI. et al. (2003) The Journal of biological chemistry 278, 36373-9. PMID: 12783892

2. Yoon, Y. et al. (2003) Molecular and cellular biology 23, 5409-20. PMID: 12861026

3. Stojanovski, D. et al. (2004) Journal of cell science 117, 1201-10. PMID: 14996942

4. Losón, OC. et al. (2013) Molecular biology of the cell 24, 659-67. PMID: 23283981

5. Yu, T. et al. (2005) Journal of cell science 118, 4141-51. PMID: 16118244

6. Koirala, S. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, E1342-51. PMID: 23530241

7. Shen, Q. et al. (2014) Molecular biology of the cell 25, 145-59. PMID: 24196833

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-FIS1 polyclonal antibody at 1ug/mL on recombinant FIS1 (Catalog No. HV072012).
Western blot

Western blot analysis was performed using anti-FIS1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: PC-12 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse heart lysate
Lane 8: Mouse spleen lysate
Lane 9: Rat brain lysate
Lane 10: Rat heart lysate
Lane11: Rat spleen lysate

References

Datasheet

Anti-FIS1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-FIS1 Polyclonal Antibody (HV072014)

Overview

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References

Datasheet

SDS

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