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Anti-FLI1 Polyclonal Antibody (HX971014)

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Anti-FLI1 Polyclonal Antibody
Anti-FLI1 Polyclonal Antibody
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Overview
Catalog No.HX971014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human FLI1 (Met1-Glu196).
Target Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
Purification Purified by antigen affinity column.
Accession Q01543
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Friend leukemia integration 1 transcription factor (FLI1) is a ~50 kDa protein. Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

1. Stockley, J. et al. (2013) Blood 122, 4090-3. PMID: 24100448
2. Stevenson, WS. et al. (2015) Blood 126, 2027-30. PMID: 26316623
3. Saultier, P. et al. (2017) Haematologica 102, 1006-1016. PMID: 28255014
Note For research use only.
Images
  • Anti-FLI1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-FLI1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: HL60 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: U937 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: K562 cell lysate
    Lane 6: Mouse thymus lysate
    Lane 7: Mouse lung lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-FLI1 Polyclonal Antibody

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