Anti-FPR1 Polyclonal Antibody (HB008014)

Name: Anti-FPR1 Polyclonal Antibody
Brand: abinScience
SKU: HB008014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HB008014

Description

Anti-FPR1 Polyclonal Antibody (HB008014) is a rabbit polyclonal antibody detecting FPR1 in ELISA, IHC, WB. Suitable for Human, Pan troglodytes, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human FPR1 (Arg163-Val242).

Target

fMet-Leu-Phe receptor, fMLP receptor, N-formyl peptide receptor, FPR, FPR1, N-formylpeptide chemoattractant receptor

Purification

Purified by antigen affinity column.

Accession

P21462

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

N-formyl peptide receptor 1 (FPR1) is a ~38 kDa protein. Pattern recognition G-protein coupled receptor (PRR/GPCR) involved in innate recognition of N-formyl-methionyl peptides derived from invading microbes and host mitochondria as pathogen- and damage-associated molecular patterns (PAMPs and DAMPs). Functions as a sensor of PAMPs and DAMPs released upon microbial infection or tissue damage, triggering immune cell activation and chemotaxis to eliminate pathogens and restore tissue homeostasis. Peptide binding leads to conformational changes coupled to heterotrimeric G(i) protein signaling. Upon GDP to GTP conversion, G(i)-alpha subunit dissociates from G-beta and G-gamma subunits. Free G(i)-alpha subunit inhibits cyclic adenylate cyclase and cAMP synthesis whereas the G-beta and G-gamma dimer activates downstream phospholipase C-beta and phosphoinositide 3-kinase signaling cascades leading to Ca(2+) influx.

1. Cooray, SN. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 18232-7. PMID: 24108355

2. Bufe, B. et al. (2015) The Journal of biological chemistry 290, 7369-87. PMID: 25605714

3. Zhuang, Y. et al. (2022) Nature communications 13, 1054. PMID: 35217703

4. Chen, G. et al. (2022) Nature communications 13, 5232. PMID: 36064945

5. Maestes, DC. et al. (1999) The Journal of biological chemistry 274, 29791-5. PMID: 10514456

6. Postma, B. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 6994-7001. PMID: 15153520

7. Murphy, PM. et al. (1991) The Journal of biological chemistry 266, 12560-7. PMID: 1712023

Note

For research use only.

Images

References

Datasheet

Anti-FPR1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-FPR1 Polyclonal Antibody (HB008014)

Overview

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References

Datasheet

SDS

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