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Anti-FPV Capsid protein VP1/VP2 Polyclonal Antibody (VK804014)

Anti-FPV Capsid protein VP1/VP2 Polyclonal Antibody
Anti-FPV Capsid protein VP1/VP2 Polyclonal Antibody
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Overview
Catalog No.VK804014
Description
Anti-FPV Capsid protein VP1/VP2 Polyclonal Antibody (VK804014) is a rabbit polyclonal antibody detecting Capsid protein VP1 in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityFeline panleukopenia virus (FPV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant FPV Capsid protein VP1 (Val181-Asp321).
Target Capsid protein VP1, Coat protein VP1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P04864
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Capsid protein VP1 is a ~80 kDa protein. Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus.

Note For research use only.
Images
  • Anti-FPV Capsid protein VP1/VP2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-FPV-VP1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant FPV Capsid protein VP1/VP2 (Catalog No: VK804012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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