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Anti-GATA1 Polyclonal Antibody (HB017024)

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Overview
Catalog No.HB017024
Description
Anti-GATA1 Polyclonal Antibody (HB017024) is a rabbit polyclonal antibody detecting Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein; GATA1; ERYF1; GF1 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, Frog, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human GATA1 (Ala211-Asp300).
Target Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein; GATA1; ERYF1; GF1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P15976, P43429, P17679, P23767, P17678, P23768, P23769, Q924Y4, O09100, P23824
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Erythroid transcription factor (GATA1) is a ~42 kDa protein. Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.

1. Ludwig, LS. et al. (2022) Blood 139, 2534-2546. PMID: 35030251
2. Mizuta, S. et al. (2014) Genes to cells : devoted to molecular & cellular mechanisms 19, 28-51. PMID: 24245781
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-GATA1 Polyclonal Antibody

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