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Anti-GFP Recombinant Antibody (N86/20) (ZP144063)

Anti-GFP Recombinant Antibody (N86/20)
Anti-GFP Recombinant Antibody (N86/20)
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Overview
Catalog No.ZP144063
Species reactivityAequorea victoria
ApplicationsELISA, IHC, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDN86/20
Clonality Monoclonal
Target Green fluorescent protein, GFP
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P42212
RRID

Anti-GFP Antibody (N86/20) (abinScience Cat# ZP144063, RRID:AB_3729094)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Green fluorescent protein (GFP) is a ~26 kDa protein. Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Note For research use only.
Images
  • Anti-GFP Recombinant Antibody (N86/20)

    SDS-PAGE

    SDS-PAGE for Anti-GFP Antibody (N86/20)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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