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Anti-GINS1 Polyclonal Antibody (HA800014)

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Overview
Catalog No.HA800014
Description
Anti-GINS1 Polyclonal Antibody (HA800014) is a rabbit polyclonal antibody detecting DNA replication complex GINS protein PSF1; GINS complex subunit 1; GINS1; KIAA0186; PSF1 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Frog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human GINS1 (Met1-Ser196).
Target DNA replication complex GINS protein PSF1; GINS complex subunit 1; GINS1; KIAA0186; PSF1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q14691, A4IFH4, Q9CZ15, Q7ZT47
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA replication complex GINS protein PSF1 (GINS1) is a ~22 kDa protein. Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.

1. Kamada, K. et al. (2007) Nature structural & molecular biology 14, 388-96. PMID: 17417653
2. Cottineau, J. et al. (2017) The Journal of clinical investigation 127, 1991-2006. PMID: 28414293
3. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425
4. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004
5. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328
6. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232
Note For research use only
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Datasheet
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Formula
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Anti-GINS1 Polyclonal Antibody

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