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Anti-GLUD1 Polyclonal Antibody (HF014014)

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Overview
Catalog No.HF014014
Description
Anti-GLUD1 Polyclonal Antibody (HF014014) is a rabbit polyclonal antibody detecting GLUD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Chicken, and Hylobates lar.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GLUD1 (Asp334-Thr556).
Target GLUD1, GDH 1, Glutamate dehydrogenase 1, mitochondrial, GLUD
Purification Purified by antigen affinity column.
Accession P00367
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glutamate dehydrogenase 1, mitochondrial (GLUD1) is a ~61 kDa protein. Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.

1. Plaitakis, A. et al. (2000) Journal of neurochemistry 75, 1862-9. PMID: 11032875
2. Smith, TJ. et al. (2001) Journal of molecular biology 307, 707-20. PMID: 11254391
3. Choi, MM. et al. (2005) FEBS letters 579, 4125-30. PMID: 16023112
4. Haigis, MC. et al. (2006) Cell 126, 941-54. PMID: 16959573
5. MacMullen, C. et al. (2001) The Journal of clinical endocrinology and metabolism 86, 1782-7. PMID: 11297618
6. Stanley, CA. et al. (1998) The New England journal of medicine 338, 1352-7. PMID: 9571255
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-GLUD1 Polyclonal Antibody

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