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Anti-GLUT4/SLC2A4 Polyclonal Antibody (HB893014)

Anti-GLUT4/SLC2A4 Polyclonal Antibody
Anti-GLUT4/SLC2A4 Polyclonal Antibody
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Overview
Catalog No.HB893014
Description
Anti-GLUT4/SLC2A4 Polyclonal Antibody (HB893014) is a rabbit polyclonal antibody detecting SLC2A4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GLUT4/SLC2A4 (Cys223-Leu278).
Target Solute carrier family 2, facilitated glucose transporter member 4, GLUT-4, GLUT4, SLC2A4, Glucose transporter type 4, insulin-responsive
Purification Purified by antigen affinity column.
Accession P14672
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Solute carrier family 2, facilitated glucose transporter member 4 (GLUT4/SLC2A4) is a ~54 kDa protein. Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.

Note For research use only.
Images
  • Anti-GLUT4/SLC2A4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GLUT4/SLC2A4 polyclonal antibody at 1ug/mL on recombinant GLUT4/SLC2A4 (Catalog No. HB893012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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