Anti-GOT1 Polyclonal Antibody (HB695014)

Name: Anti-GOT1 Polyclonal Antibody
Brand: abinScience
SKU: HB695014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB695014

Description

Anti-GOT1 Polyclonal Antibody (HB695014) is a rabbit polyclonal antibody detecting GOT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Equus caballus, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human GOT1 (Met1-Gln413).

Target

GOT1, Cysteine aminotransferase, cytoplasmic, Aspartate aminotransferase, cytoplasmic, Glutamate oxaloacetate transaminase 1, Cysteine transaminase, cytoplasmic, Transaminase A, cAspAT, cCAT

Purification

Purified by antigen affinity column.

Accession

P17174

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Aspartate aminotransferase, cytoplasmic (GOT1) is a ~46 kDa protein. Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide.

1. Shen, H. et al. (2011) Journal of human genetics 56, 801-5. PMID: 21900944

2. Irino, Y. et al. (2016) Scientific reports 6, 36749. PMID: 27827456

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GOT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse heart lysate
Lane 3: Mouse liver lysate
Lane 4: Rat brain lysate
Lane 5: Rat heart lysate
Lane 6: Rat liver lysate

References

Datasheet

Anti-GOT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GOT1 Polyclonal Antibody (HB695014)

Overview

Images

References

Datasheet

SDS

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