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Anti-GPR183 Polyclonal Antibody (HB793014)

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Overview
Catalog No.HB793014
Description
Anti-GPR183 Polyclonal Antibody (HB793014) is a rabbit polyclonal antibody detecting G-protein coupled receptor 183; Epstein-Barr virus-induced G-protein coupled receptor 2; EBI2; EBV-induced G-protein coupled receptor 2; hEBI2; GPR183; EBI2 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human GPR183 (Lys313-Lys361).
Target G-protein coupled receptor 183; Epstein-Barr virus-induced G-protein coupled receptor 2; EBI2; EBV-induced G-protein coupled receptor 2; hEBI2; GPR183; EBI2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P32249, Q1RMI1, Q3U6B2, D4A7K7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

G-protein coupled receptor 183 (GPR183) is a ~41 kDa protein. G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes. Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions.

1. Hannedouche, S. et al. (2011) Nature 475, 524-7. PMID: 21796212
2. Benned-Jensen, T. et al. (2012) The Journal of biological chemistry 287, 35470-35483. PMID: 22875855
3. Zhang, L. et al. (2012) Molecular pharmacology 82, 1094-103. PMID: 22930711
4. Barroso, R. et al. (2012) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26, 4841-54. PMID: 22913878
5. Rutkowska, A. et al. (2015) Glia 63, 341-51. PMID: 25297897
6. Benned-Jensen, T. et al. (2011) The Journal of biological chemistry 286, 29292-29302. PMID: 21673108
Note For research use only
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Anti-GPR183 Polyclonal Antibody

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