Anti-GRSF1 Polyclonal Antibody (HB421014)

Name: Anti-GRSF1 Polyclonal Antibody
Brand: abinScience
SKU: HB421014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB421014

Description

Anti-GRSF1 Polyclonal Antibody (HB421014) is a rabbit polyclonal antibody detecting G-rich sequence factor 1; GRSF-1; GRSF1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human GRSF1 (Val149-Ser335).

Target

G-rich sequence factor 1; GRSF-1; GRSF1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q12849, Q8C5Q4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

G-rich sequence factor 1 (GRSF1) is a ~53 kDa protein. Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome.

1. Antonicka, H. et al. (2013) Cell metabolism 17, 386-98. PMID: 23473033

2. Jourdain, AA. et al. (2013) Cell metabolism 17, 399-410. PMID: 23473034

3. Pietras, Z. et al. (2018) Nature communications 9, 2558. PMID: 29967381

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-GRSF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate

References

Datasheet

Anti-GRSF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GRSF1 Polyclonal Antibody (HB421014)

Overview

Images

References

Datasheet

SDS