Anti-GSK3B Polyclonal Antibody (HW521014)

Name: Anti-GSK3B Polyclonal Antibody
Brand: abinScience
SKU: HW521014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW521014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GSK3B (Met1-Thr420).

Target

Glycogen synthase kinase-3 beta, GSK3B, Serine/threonine-protein kinase GSK3B, GSK-3 beta

Purification

Purified by antigen affinity column.

Accession

P49841

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glycogen synthase kinase-3 beta (GSK3B) is a ~46 kDa protein. Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase.

1. Frame, S. et al. (2001) Molecular cell 7, 1321-7. PMID: 11430833

2. Dajani, R. et al. (2003) The EMBO journal 22, 494-501. PMID: 12554650

3. Cho, JH. et al. (2004) Journal of neurochemistry 88, 349-58. PMID: 14690523

4. Yin, L. et al. (2006) Science (New York, N.Y.) 311, 1002-5. PMID: 16484495

5. Boyle, WJ. et al. (1991) Cell 64, 573-84. PMID: 1846781

6. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854

7. Beals, CR. et al. (1997) Science (New York, N.Y.) 275, 1930-4. PMID: 9072970

8. Welsh, GI. et al. (1993) The Biochemical journal 294 ( Pt 3), 625-9. PMID: 8397507

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GSK3B polyclonal antibody at 1ug/mL on recombinant GSK3B (Catalog No. HW521012).

References

Datasheet

Anti-GSK3B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GSK3B Polyclonal Antibody (HW521014)

Overview

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References

Datasheet

SDS

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