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Anti-GSTA1 Polyclonal Antibody (HY197014)

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Overview
Catalog No.HY197014
Description
Anti-GSTA1 Polyclonal Antibody (HY197014) is a rabbit polyclonal antibody detecting GSTA1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GSTA1 (Ala2-Phe222).
Target GSTA1, GTH1, GST HA subunit 1, 13-hydroperoxyoctadecadienoate peroxidase, GSTA1-1, Glutathione S-transferase A1, Androst-5-ene-3, 17-dione isomerase, GST class-alpha member 1, GST-epsilon
Purification Purified by antigen affinity column.
Accession P08263
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glutathione S-transferase A1 (GSTA1) is a ~25 kDa protein. Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid.

1. Bogaards, JJ. et al. (1997) Chemical research in toxicology 10, 310-7. PMID: 9084911
2. Pettersson, PL. et al. (2001) The Journal of biological chemistry 276, 11698-704. PMID: 11152686
3. Seeley, SK. et al. (2006) Biochimica et biophysica acta 1760, 1064-70. PMID: 16624487
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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