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Anti-GSTM4 Polyclonal Antibody (HX884014)

Anti-GSTM4 Polyclonal Antibody
Anti-GSTM4 Polyclonal Antibody
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Overview
Catalog No.HX884014
Description
Anti-GSTM4 Polyclonal Antibody (HX884014) is a rabbit polyclonal antibody detecting GSTM4 in ELISA, IHC, WB. Suitable for Mouse, Human, Macaca fascicularis, and Rat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GSTM4 (Arg18-Trp215).
Target GSTM4-4, Glutathione S-transferase Mu 4, GSTM4, GST-Mu2, Leukotriene C4 synthase GSTM4, GST class-mu 4
Purification Purified by antigen affinity column.
Accession Q03013
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glutathione S-transferase Mu 4 (GSTM4) is a ~25 kDa protein. Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the conjugation of leukotriene A4 with reduced glutathione (GSH) to form leukotriene C4. Can also catalyze the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions.

1. Comstock, KE. et al. (1994) Archives of biochemistry and biophysics 311, 487-95. PMID: 8203914
2. Ross, VL. et al. (1993) The Biochemical journal 294 ( Pt 2), 373-80. PMID: 8373352
3. Dalli, J. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, 12232-12237. PMID: 27791009
Note For research use only.
Images
  • Anti-GSTM4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GSTM4 polyclonal antibody at 1ug/mL on recombinant GSTM4 (Catalog No. HX884012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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