Anti-GTF2E2 Polyclonal Antibody (HA614014)

Name: Anti-GTF2E2 Polyclonal Antibody
Brand: abinScience
SKU: HA614014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HA614014

Description

Anti-GTF2E2 Polyclonal Antibody (HA614014) is a rabbit polyclonal antibody detecting Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2; GTF2E2; TF2E2 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Frog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human GTF2E2 (Ser68-Ser243).

Target

Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2; GTF2E2; TF2E2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P29084, Q2KJF9, Q9D902, P29540

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription initiation factor IIE subunit beta (GTF2E2) is a ~33 kDa protein. Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-GTF2E2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A375 cell lysate
Lane 2: Jurkat cell lysate

References

Datasheet

Anti-GTF2E2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GTF2E2 Polyclonal Antibody (HA614014)

Overview

Images

References

Datasheet

SDS