Anti-H1-1 Polyclonal Antibody (HB348014)

Name: Anti-H1-1 Polyclonal Antibody
Brand: abinScience
SKU: HB348014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HB348014

Description

Anti-H1-1 Polyclonal Antibody (HB348014) is a rabbit polyclonal antibody detecting Histone H1.1; Histone H1a; H1-1; H1F1; HIST1H1A in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, Mouse, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human H1-1 (Ala39-Glu118).

Target

Histone H1.1; Histone H1a; H1-1; H1F1; HIST1H1A

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q02539, G3N131, D4A3K5, P43275, P16402, P43276, P09987, P16403, D3ZBN0, P43277, P10412, P15865, P43274

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Histone H1.1 (H1-1) is a ~21 kDa protein. Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex. Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

1. Lever, MA. et al. (2000) Nature 408, 873-6. PMID: 11130728

Note

For research use only

Images

References

Datasheet

Anti-H1-1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-H1-1 Polyclonal Antibody (HB348014)

Overview

Images

References

Datasheet

SDS

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