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Anti-HADH Polyclonal Antibody (HS743014)

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Overview
Catalog No.HS743014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human HADH (Met1-Arg236).
Target HCDH, HAD, Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial, HADH, SCHAD, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, HAD1, Short-chain 3-hydroxyacyl-CoA dehydrogenase, HADHSC
Purification Purified by antigen affinity column.
Accession Q16836
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH) is a ~34 kDa protein. Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion. Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes.

1. Barycki, JJ. et al. (1999) Biochemistry 38, 5786-98. PMID: 10231530
2. Clayton, PT. et al. (2001) The Journal of clinical investigation 108, 457-65. PMID: 11489939
3. Bennett, MJ. et al. (2006) Molecular genetics and metabolism 89, 74-9. PMID: 16725361
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-HADH Polyclonal Antibody

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