Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014)

Name: Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody
Brand: abinScience
SKU: VK734014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

VK734014

Description

Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014) is a rabbit polyclonal antibody detecting Shutoff protein in ELISA, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.

Species reactivity

Human adenovirus B serotype 7 (HAdV-7)

Applications

ELISA, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HAdV-7 Shutoff protein/L4 (Leu373-Arg537).

Target

Shutoff protein, 100 kDa protein, p100K, 100K-chaperone protein, L4-100K, Shutoff protein 100K, L4

Purification

Purified by antigen affinity column.

Accession

Q5EY60

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Shutoff protein (L4) is a ~92 kDa protein. Protein that inhibits host translation while promoting late viral translation by ribosome shunting. Blocks host cap-dependent translation by binding to eIF4G, displacing MKNK1 from cap initiation complexes and preventing EIF4E phosphorylation. Binds to the tripartite leader sequence of viral late mRNAs and recruits host eIF4G, PABPC1/poly-A binding protein and 40S ribosomes subunits on viral mRNAs, allowing ribosome shunting and efficient translation of late viral mRNAs even though conventional translation via ribosome scanning from the cap has been shut off in the host cell. During assembly, acts as a chaperone protein that helps hexon proteins assembly into trimers.

Note

For research use only.

Images

References

Datasheet

Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HAdV-7 Shutoff protein/L4 Polyclonal Antibody (VK734014)

Overview

Images

References

Datasheet

SDS

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