Anti-HAO1 Polyclonal Antibody (RD448014)

Name: Anti-HAO1 Polyclonal Antibody
Brand: abinScience
SKU: RD448014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

RD448014

Description

Anti-HAO1 Polyclonal Antibody (RD448014) is a rabbit polyclonal antibody detecting HAO1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Rat HAO1 (Asp61-Ile370).

Target

2-Hydroxyacid oxidase 1, EC:1.1.3.15, EC:1.2.3.5, GO, GOX, GOX1, Glycolate oxidase, Glyoxylate oxidase, HAO1, HAOX1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

B0BNF9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

B0BNF9

Background

2-Hydroxyacid oxidase 1 (HAO1) is a ~40 kDa protein. Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate. The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones. Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate. Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-HAO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Rat liver lysate

References

Datasheet

Anti-HAO1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HAO1 Polyclonal Antibody (RD448014)

Overview

Images

References

Datasheet

SDS

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