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Anti-HARS2 Polyclonal Antibody (HA656014)

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Overview
Catalog No.HA656014
Description
Anti-HARS2 Polyclonal Antibody (HA656014) is a rabbit polyclonal antibody detecting mitochondrial; 6.1.1.21; Histidine--tRNA ligase-like; Histidyl-tRNA synthetase; HisRS; HARS2; HARSL; HARSR; HO3 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human HARS2 (Ala90-Ser506).
Target Histidine--tRNA ligase, mitochondrial; 6.1.1.21; Histidine--tRNA ligase-like; Histidyl-tRNA synthetase; HisRS; HARS2; HARSL; HARSR; HO3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P49590, A5D7V9, Q99KK9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Histidine--tRNA ligase, mitochondrial (HARS2) is a ~56 kDa protein. Mitochondrial aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP).

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-HARS2 Polyclonal Antibody

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