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Anti-HAS2 Polyclonal Antibody (HV192014)

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Overview
Catalog No.HV192014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human HAS2 (Glu67-Thr172).
Target HA synthase 2, Hyaluronan synthase 2, Hyaluronic acid synthase 2, HAS2, Hyaluronate synthase 2
Purification Purified by antigen affinity column.
Accession Q92819
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Hyaluronan synthase 2 (HAS2) is a ~63 kDa protein. Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer (Probable). Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan.

1. Karousou, E. et al. (2010) The Journal of biological chemistry 285, 23647-54. PMID: 20507985
2. Vigetti, D. et al. (2011) The Journal of biological chemistry 286, 7917-7924. PMID: 21228273
3. Rilla, K. et al. (2013) The Journal of biological chemistry 288, 5973-83. PMID: 23303191
4. Kasai, K. et al. (2020) Biochemical and biophysical research communications 533, 732-738. PMID: 32993960
5. Watanabe, K. et al. (1996) The Journal of biological chemistry 271, 22945-8. PMID: 8798477
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-HAS2 Polyclonal Antibody

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