Anti-HBoV1 Initiator protein NS1 Polyclonal Antibody (VK140024)

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Datasheet

Overview

Catalog No.

VK140024

Description

Anti-HBoV1 Initiator protein NS1 Polyclonal Antibody (VK140024) is a rabbit polyclonal antibody detecting Initiator protein NS1. Suitable for Primate bocaparvovirus 1.

Species reactivity

Primate bocaparvovirus 1

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HBoV1 Initiator protein NS1 (Met1-Gly271).

Target

EC:3.1.21.-, EC:3.6.4.12, Initiator protein NS1, NS1, Non-structural protein 1, Non-structural protein NS1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

D0EZM8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Initiator protein NS1 is a ~88 kDa protein. Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis. The helicase activity unwinds DNA in a 3'-5' direction on the longer strand.

Note

For research use only

Images

References

Datasheet

Anti-HBoV1 Initiator protein NS1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HBoV1 Initiator protein NS1 Polyclonal Antibody (VK140024)

Overview

Images

References

Datasheet

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