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Anti-HBV HBsAg Polyclonal Antibody (VK539024)

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Overview
Catalog No.VK539024
Description
Anti-HBV HBsAg Polyclonal Antibody (VK539024) is a rabbit polyclonal antibody detecting L-HBsAg. Suitable for Hepatitis B virus genotype B1, Hepatitis B virus genotype B2 subtype adw, Hepatitis B virus genotype B1 subtype adw, Hepatitis B virus genotype B2, and Hepatitis B virus genotype B/C subtype adw.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHepatitis B virus (HBV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant HBV HBsAg (Met1-Arg253).
Target L glycoprotein, L-HBsAg, LHB, Large S protein, Large envelope protein, Large surface protein, Major surface antigen, S
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q67926
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Large envelope protein (HBsAg) is a ~43 kDa protein. The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-HBV HBsAg Polyclonal Antibody

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