Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5) (VK665043)

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Overview

Catalog No.

VK665043

Description

Anti-HCMV/HHV5 gB/Envelope glycoprotein B Antibody (KE5) [KE5] (VK665043) is a human monoclonal antibody detecting Envelope glycoprotein B in ELISA, WB.

Species reactivity

Human cytomegalovirus (HHV-5) (Human herpesvirus 5)

Applications

ELISA, WB

Host species

Human

Isotype

IgG1, kappa

Clone ID

KE5

Clonality

Monoclonal

Target

Envelope glycoprotein B, gB, gB

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P13201, F5HB53, P06473

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Envelope glycoprotein B (gB) is a ~101 kDa protein. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. May be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Interacts with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HCMV/HHV5 Envelope glycoprotein B/gB(Catalog No: VK665011)
Lane 2: negative control
Lane 3: negative control
Bioactivity

Detects recombinant HCMV gB protein (Catalog No: VK665011) in indirect ELISA

References

Datasheet

Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HCMV/HHV5 gB/Envelope glycoprotein B Recombinant Antibody (KE5) (VK665043)

Overview

Images

References

Datasheet

SDS

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