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Anti-HCoV-229E NP/Nucleoprotein Polyclonal Antibody (VK612014)

Anti-HCoV-229E NP/Nucleoprotein Polyclonal Antibody
Anti-HCoV-229E NP/Nucleoprotein Polyclonal Antibody
Price(USD): $
Spec:
  • 50ug
  • 100ug
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  • Datasheet

Overview
Catalog No.VK612014
Species reactivityHuman coronavirus 229E (HCoV-229E)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant HCoV-229E NP/Nucleoprotein (Met1-Asn389).
Target Nucleoprotein, Nucleocapsid protein, NC, Protein N, N, 7
Purification Purified by antigen affinity column.
Accession P15130
RRID Anti-HCoV-229E NP/Nucleoprotein Polyclonal Antibody (abinScience Cat# VK612014, RRID:AB_3723637)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleoprotein (NP) is a ~43 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Note For research use only.
Images
  • Anti-HCoV-229E NP/Nucleoprotein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HCoV-229E NP/Nucleoprotein polyclonal antibody at 1ug/mL on recombinant HCoV-229E NP/Nucleoprotein (Catalog No. VK612012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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