Anti-HCoV-229E Spike Protein Recombinant Antibody (SAA2363) (VK666013)

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50ug
100ug
1mg

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Datasheet
SDS

Overview

Catalog No.

VK666013

Description

Anti-HCoV-229E Spike Protein Antibody (SAA2363) [SAA2363] (VK666013) is a human monoclonal antibody detecting Spike glycoprotein in ELISA, Neutralization. Suitable for HCoV-229E.

Species reactivity

HCoV-229E

Applications

ELISA, Neutralization

Host species

Human

Isotype

IgG

Clone ID

SAA2363

Clonality

Monoclonal

Target

Spike glycoprotein, S glycoprotein, E2, Peplomer protein, S

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P15423

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Spike glycoprotein is a ~128 kDa protein. S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-HCoV-229E Spike Protein Antibody (SAA2363)

References

Datasheet

Anti-HCoV-229E Spike Protein Recombinant Antibody (SAA2363).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HCoV-229E Spike Protein Recombinant Antibody (SAA2363) (VK666013)

Overview

Images

References

Datasheet

SDS

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