Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014)

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Spec:

50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

VK457014

Description

Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Bovine coronavirus and Equine coronavirus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human coronavirus OC43, Bovine coronavirus, Porcine hemagglutinating encephalomyelitis virus, Equine coronavirus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HCoV-OC43 NP/Nucleoprotein (Met1-Ile448).

Target

N, NC, Nucleocapsid protein, Nucleoprotein, Protein N

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P33469

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoprotein (NP) is a ~49 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-NCAP-CVHOC polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HCoV-OC43 NP/Nucleoprotein (Catalog No: VK457012)

References

Datasheet

Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014)

Overview

Images

References

Datasheet

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