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Anti-HDAC5 Polyclonal Antibody (HV143014)

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Overview
Catalog No.HV143014
Description
Anti-HDAC5 Polyclonal Antibody (HV143014) is a rabbit polyclonal antibody detecting HDAC5 in ELISA, IHC, WB. Suitable for Human, Mouse, Cricetulus griseus, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human HDAC5 (Phe681-Ser1087).
Target Histone deacetylase 5, HDAC5, HD5, KIAA0600, Antigen NY-CO-9
Purification Purified by antigen affinity column.
Accession Q9UQL6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Histone deacetylase 5 (HDAC5) is a ~121 kDa protein. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.

1. Lee, DY. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 2072-2077. PMID: 28167758
Note For research use only.
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Datasheet
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Formula
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Formula
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V₁ (Stock Vol.)
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Working Solution
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Anti-HDAC5 Polyclonal Antibody

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