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Anti-HERPUD1 Polyclonal Antibody (HC651024)

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Overview
Catalog No.HC651024
Description
Anti-HERPUD1 Polyclonal Antibody (HC651024) is a rabbit polyclonal antibody detecting Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein; Methyl methanesulfonate (MMF)-inducible fragment protein 1; HERPUD1; HERP; KIAA0025; MIF1 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human HERPUD1 (Glu8-Asn87).
Target Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein; Methyl methanesulfonate (MMF)-inducible fragment protein 1; HERPUD1; HERP; KIAA0025; MIF1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q15011, Q9JJK5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1) is a ~43 kDa protein. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Could enhance presenilin-mediated amyloid-beta protein 40 generation. Binds to ubiquilins and this interaction is required for efficient degradation of CD3D via the ERAD pathway.

1. Schulze, A. et al. (2005) Journal of molecular biology 354, 1021-7. PMID: 16289116
2. Schulz, J. et al. (2017) Journal of cell science 130, 3322-3335. PMID: 28827405
3. Kim, TY. et al. (2008) Biochemical and biophysical research communications 369, 741-6. PMID: 18307982
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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