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Anti-HHV-8 HELI Polyclonal Antibody (VK084014)

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Overview
Catalog No.VK084014
Description
Anti-HHV-8 HELI Polyclonal Antibody (VK084014) is a rabbit polyclonal antibody detecting DNA replication helicase; 3.6.4.-; HELI; ORF44 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman herpesvirus 8 type P (HHV-8)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant HHV-8 HELI (Gln246-Phe647).
Target DNA replication helicase; 3.6.4.-; HELI; ORF44
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q2HR89
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA replication helicase is a ~88 kDa protein. Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Possesses helicase-like motifs and therefore may act as the helicase subunit of the complex.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-HHV-8 HELI Polyclonal Antibody

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