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Anti-HIC1 Polyclonal Antibody (HA798014)

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Overview
Catalog No.HA798014
Description
Anti-HIC1 Polyclonal Antibody (HA798014) is a rabbit polyclonal antibody detecting Hypermethylated in cancer 1 protein; Hic-1; Zinc finger and BTB domain-containing protein 29; HIC1; ZBTB29 in ELISA, IHC, WB. Suitable for Human, Mouse, and Zebrafish.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human HIC1 (Lys561-Thr733).
Target Hypermethylated in cancer 1 protein; Hic-1; Zinc finger and BTB domain-containing protein 29; HIC1; ZBTB29
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q14526, Q9R1Y5, Q9JLZ6, Q90W33, Q96JB3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Hypermethylated in cancer 1 protein (HIC1) is a ~76 kDa protein. Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses.

1. Deltour, S. et al. (2002) Molecular and cellular biology 22, 4890-901. PMID: 12052894
2. Pinte, S. et al. (2004) The Journal of biological chemistry 279, 38313-24. PMID: 15231840
3. Zhang, W. et al. (2010) Oncogene 29, 2467-76. PMID: 20154726
4. Chen, WY. et al. (2005) Cell 123, 437-48. PMID: 16269335
5. Van Rechem, C. et al. (2010) Molecular and cellular biology 30, 4045-59. PMID: 20547755
6. Valenta, T. et al. (2006) The EMBO journal 25, 2326-37. PMID: 16724116
7. Briggs, KJ. et al. (2008) Genes & development 22, 770-85. PMID: 18347096
Note For research use only
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Datasheet
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Formula
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Anti-HIC1 Polyclonal Antibody

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