Anti-HIV-1 rev/ART/TRS protein Recombinant Nanobody (SAA1422) (VK497013)

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Overview

Catalog No.

VK497013

Description

Anti-HIV-1 rev/ART/TRS protein Nanobody (SAA1422) [SAA1422] (VK497013) is a alpaca monoclonal antibody detecting ART/TRS in ELISA, IF.

Species reactivity

Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1)

Applications

ELISA, IF

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA1422

Clonality

Monoclonal

Target

Protein Rev, ART/TRS, Anti-repression transactivator, Regulator of expression of viral proteins, rev

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P04618

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein Rev (ART/TRS) is a ~13 kDa protein. Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-HIV-1 rev/ART/TRS protein Nanobody (SAA1422)

References

Datasheet

Anti-HIV-1 rev/ART/TRS protein Recombinant Nanobody (SAA1422).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HIV-1 rev/ART/TRS protein Recombinant Nanobody (SAA1422) (VK497013)

Overview

Images

References

Datasheet

SDS

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