Anti-HIV-1 Vif/SOR protein Recombinant Nanobody (SAA1421) (VK454013)

Name: Anti-HIV-1 Vif/SOR protein Recombinant Nanobody (SAA1421)
Brand: abinScience
SKU: VK454013
Price: 191 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK454013

Description

Anti-HIV-1 Vif/SOR protein Nanobody (SAA1421) [SAA1421] (VK454013) is a alpaca monoclonal antibody detecting SOR protein in ELISA, IP.

Species reactivity

Human immunodeficiency virus type 1 group M subtype B (isolate NY5) (HIV-1)

Applications

ELISA, IP

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA1421

Clonality

Monoclonal

Target

Virion infectivity factor, Vif, SOR protein, p17, p7, vif

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P12504

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Virion infectivity factor (Vif/SOR protein) is a ~22 kDa protein. Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G by promoting their ubiquitination and degradation. Acts as a substrate recognition component of an E3 ubiquitin-protein ligase complex: mechanistically, Vif hijacks a host cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) and the transcription coactivator CBFB/CBF-beta to form an active E3 ubiquitin-protein ligase complex that targets APOBEC3G and APOBEC3F for polyubiquitination, leading to their degradation by the proteasome. The complex can also ubiquitinate host APOBEC3H to some extent. Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism. May interact directly with APOBEC3G mRNA in order to inhibit its translation.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-HIV-1 Vif/SOR protein Nanobody (SAA1421)

References

Datasheet

Anti-HIV-1 Vif/SOR protein Recombinant Nanobody (SAA1421).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HIV-1 Vif/SOR protein Recombinant Nanobody (SAA1421) (VK454013)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us