Anti-HMBS Polyclonal Antibody (HY082014)

Name: Anti-HMBS Polyclonal Antibody
Brand: abinScience
SKU: HY082014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HY082014

Description

Anti-HMBS Polyclonal Antibody (HY082014) is a rabbit polyclonal antibody detecting HMBS in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HMBS (Leu85-Ser337).

Target

Hydroxymethylbilane synthase, PBG-D, Pre-uroporphyrinogen synthase, Porphobilinogen deaminase, PBGD, UPS, HMBS

Purification

Purified by antigen affinity column.

Accession

P08397

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Porphobilinogen deaminase (HMBS) is a ~39 kDa protein. As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact.

1. Cunha, L. et al. (2008) Proteins 71, 855-73. PMID: 18004775

2. Song, G. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 396-404. PMID: 18936296

3. Ulbrichova, D. et al. (2009) Blood cells, molecules & diseases 42, 167-73. PMID: 19138865

4. Bustad, HJ. et al. (2013) Bioscience reports 33. PMID: 23815679

Note

For research use only.

Images

References

Datasheet

Anti-HMBS Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HMBS Polyclonal Antibody (HY082014)

Overview

Images

References

Datasheet

SDS

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