Anti-HMGB1 Polyclonal Antibody (HY200014)

Name: Anti-HMGB1 Polyclonal Antibody
Brand: abinScience
SKU: HY200014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HY200014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human HMGB1 (Met1-Glu215).

Target

High mobility group protein B1, HMGB1, HMG1, HMG-1, High mobility group protein 1

Purification

Purified by antigen affinity column.

Accession

P09429

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

High mobility group protein B1 (HMGB1) is a ~24 kDa protein. Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as a sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy.

1. Wei, J. et al. (2021) Cell 184, 76-91.e13. PMID: 33147444

2. Avgousti, DC. et al. (2016) Nature 535, 173-7. PMID: 27362237

3. Wang, G. et al. (2021) Cell death discovery 7, 337. PMID: 34743181

4. Yang, H. et al. (2013) Journal of leukocyte biology 93, 865-73. PMID: 23446148

5. Li, G. et al. (2013) Frontiers in immunology 4, 68. PMID: 23519706

6. Li, G. et al. (2013) Seminars in cancer biology 23, 380-90. PMID: 23994764

7. Lee, SA. et al. (2014) Yonsei medical journal 55, 1165-76. PMID: 25048472

8. Min, HJ. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 1797-806. PMID: 23303669

9. Lee, LC. et al. (2014) PloS one 9, e115809. PMID: 25549101

Note

For research use only.

Images

References

Datasheet

Anti-HMGB1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HMGB1 Polyclonal Antibody (HY200014)

Overview

Images

References

Datasheet

SDS

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