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Anti-HMPV Matrix protein/M Polyclonal Antibody (VK777014)

Anti-HMPV Matrix protein/M Polyclonal Antibody
Anti-HMPV Matrix protein/M Polyclonal Antibody
Anti-HMPV Matrix protein/M Polyclonal Antibody
Anti-HMPV Matrix protein/M Polyclonal Antibody
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  • Overview

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  • Datasheet

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Overview
Catalog No.VK777014
Species reactivityHuman metapneumovirus (strain CAN97-83) (HMPV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant HMPV Matrix protein/M (Glu2-Arg254).
Target Matrix protein, M protein, M
Purification Purified by antigen affinity column.
Accession Q6WB99
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Matrix protein is a ~27 kDa protein. Plays a crucial role in virus assembly into filaments and budding. Early in infection, localizes in the nucleus where it may inhibit host cell transcription. Later in infection, traffics to the cytoplasm through the action of host CRM1 to associate with inclusion bodies, the site of viral transcription and replication. During virus assembly and budding, acts as a bridge between the nucleocapsid and the lipid bilayer.

Note For research use only.
Images
  • Anti-HMPV Matrix protein/M Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HMPV Matrix protein/M polyclonal antibody at 1ug/mL on recombinant HMPV Matrix protein/M (Catalog No. VK777012).

  • Anti-HMPV Matrix protein/M Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-M polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant HMPV Matrix protein/M (Catalog No: VK777012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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