Anti-HNRNPF Polyclonal Antibody (HB316014)

Name: Anti-HNRNPF Polyclonal Antibody
Brand: abinScience
SKU: HB316014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB316014

Description

Anti-HNRNPF Polyclonal Antibody (HB316014) is a rabbit polyclonal antibody detecting N-terminally processed; HNRNPF; HNRPF in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human HNRNPF (Met1-Asp196).

Target

Heterogeneous nuclear ribonucleoprotein F; hnRNP F; Nucleolin-like protein mcs94-1; Heterogeneous nuclear ribonucleoprotein F, N-terminally processed; HNRNPF; HNRPF

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P52597, Q9Z2X1, Q5E9J1, Q794E4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Heterogeneous nuclear ribonucleoprotein F (HNRNPF) is a ~45 kDa protein. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-HNRNPF polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: 4T1 cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: U2OS cell lysate
Lane 8: HepG2 cell lysate
Lane 9: Mouse lung lysate

References

Datasheet

Anti-HNRNPF Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HNRNPF Polyclonal Antibody (HB316014)

Overview

Images

References

Datasheet

SDS