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Anti-HP1BP3 Polyclonal Antibody (HB293014)

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Overview
Catalog No.HB293014
Description
Anti-HP1BP3 Polyclonal Antibody (HB293014) is a rabbit polyclonal antibody detecting Heterochromatin protein 1-binding protein 3; Protein HP1-BP74; HP1BP3 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, Mouse, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human HP1BP3 (Thr138-Pro424).
Target Heterochromatin protein 1-binding protein 3; Protein HP1-BP74; HP1BP3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q5SSJ5, Q08DU9, Q6P747, Q3TEA8, Q5ZM33
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Heterochromatin protein 1-binding protein 3 (HP1BP3) is a ~61 kDa protein. Component of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity. Mediates chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance and self-renewal.

1. Dutta, B. et al. (2014) Molecular & cellular proteomics : MCP 13, 2183-97. PMID: 24830416
2. Dutta, B. et al. (2014) Molecular & cellular proteomics : MCP 13, 3236-49. PMID: 25100860
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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