Anti-HPV B19 NS1/Non-structural protein 1 Polyclonal Antibody (VK472014)

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Overview

Catalog No.

VK472014

Description

Anti-HPV B19 NS1/Non-structural protein 1 Polyclonal Antibody (VK472014) is a rabbit polyclonal antibody detecting Non-structural protein NS1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human parvovirus B19 (HPV B19)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human parvovirus B19 (HPV B19) NS1/Non-structural protein 1 (Met1-Glu671).

Target

NS, Non-structural protein NS1

Purification

Purified by antigen affinity column.

Accession

Q6TV13

RRID

Anti-HPV B19 NS1/Non-structural protein 1 Polyclonal Antibody (abinScience Cat# VK472014, RRID:AB_3725086)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Initiator protein NS1 is a ~74 kDa protein. Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promoter. Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR). Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters. Transactivates several host promoters some of which induce the S cell cycle phase for the production of host replicative proteins. Up-regulates the expression of host E2F4 and E2F5 and interacts with both these factors thereby inhibiting the host cell cycle G2/M transition.

Note

For research use only.

Images

References

Datasheet

Anti-HPV B19 NS1/Non-structural protein 1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HPV B19 NS1/Non-structural protein 1 Polyclonal Antibody (VK472014)

Overview

Images

References

Datasheet

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